Some applications of ELISA are:

• ELISA can be used to diagnose infectious diseases caused by bacteria, viruses, fungi, parasites, or prions. For example, ELISA can detect the presence of antibodies or antigens for HIV/AIDS, hepatitis, tuberculosis, Lyme disease, COVID-19, and Creutzfeldt-Jakob disease.

• ELISA can be used to determine the immune status or immunity of an individual to certain diseases or vaccines. For example, ELISA can measure the levels of antibodies for measles, rubella, varicella-zoster virus, tetanus, and rabies.

• ELISA can be used to detect autoantibodies that are produced by the immune system against self-antigens in autoimmune diseases. For example, ELISA can identify autoantibodies for rheumatoid arthritis, systemic lupus erythematosus, Sjögren's syndrome, celiac disease, and myasthenia gravis.

• ELISA can be used to determine the blood type and compatibility of donors and recipients in transfusion medicine. For example, ELISA can test for blood group systems such as ABO.

• ELISA can be used to identify biological evidence and establish the identity or relationship of individuals in forensic science. For example, ELISA can perform blood typing, DNA profiling, paternity testing, and semen analysis.

• ELISA can be used in the food industry to detect the presence of allergens for legally required ingredient labeling. For example, ELISA can test for potential food allergens such as milk, peanuts, walnuts, almonds, and eggs.